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KMID : 0614619940260050825
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Korean Journal of Gastroenterology
1994 Volume.26 No. 5 p.825 ~ p.832
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Diagnosis of Hepatitis C Virus Infection in Patients with NANB Type Liver Disease Using Polymerase Chain Reaction and Second-Generation Enzyme-linked Immunosorbent Assay
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이복근
권기호/박양훈/김한성/정찬수/제영성/이준상/유방현/박영홍
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Abstract
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The first-generation enzyme-linked immumosorbent assay(ELISA) method to detect hepatitis C virus(HCV) has been used with relatively good sensitivity in the diagnosis of chronic non-A non-B hepatitis infecton, but its sensitivity in cases of acute
hepatitis remains poor. The second-generation ELIA which uses recombinant proteins and synthetic peptides from both structural and nonstructural regions increases the sensitivity in diagnosis HCV infection, and diminishes the number of
false-positive
reactions. The polymerase chain reaction(PCR) has been used to detect infectivity of anti-HCV positive patients more accurately, To evaluate vertical transmission, and to monitor the response to antiviral therapy for chronic HCV infection.
Serum samples from 35 patients with non-A non-B type liver disease were tested by the second-generation ELISA and the PCR to evaluate the roles of hepatitis C viral infections in NANB patients. HCV-RNA was detected by single polymerase chain
reaction
technique using primers deduced from the 5'noncoding-region of HCV genome. Among the 35 patients, 27 patients were positive for anti-HCV(Group A), while 8 were negative for anti-HCV(Group B).
Among anti-HCV positive patients, 11% and 33% of patients were related to the transfusion and acupuncture, respectively. We could detect HCV-RNA in the sera from 27 patients (100%) in the group A and 4 patients(50%) in the group B.
We conclude that the combination methods of second-generation ELISA plus PCR reduce the chance of false diagnosis of HCV infection compared to the first generation ELISA and the PCR can be used to diagnose HCV infection in anti-HCV negative
hepatitis
patients.(Korean J Gastroenterol 1994 ; 26 : 825-832).
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